Saint Petersburg is home to more than two hundred museums, many of them hosted in historic buildings. The largest of the museums is the Hermitage Museum, featuring interiors of the former imperial residence and a vast collection of art.

The Russian Museum is a large museum devoted to the Russian fine art specifically. The apartments of some famous Petersburgers, including Alexander Pushkin, Fyodor Dostoevsky, Nikolai Rimsky-Korsakov, Feodor Chaliapin, Alexander Blok, Vladimir Nabokov, Anna Akhmatova, Mikhail Zoshchenko, Joseph Brodsky, as well as some palace and park ensembles of the southern suburbs and notable architectural monuments such as St. Isaac's Cathedral, have also been turned into public museums.

The Kunstkamera, with its collection established in 1714 by Peter the Great to collect curiosities from all over the world, is sometimes considered the first museum in Russia, which has evolved into the present-day Peter the Great Museum of Anthropology and Ethnography. The Russian Ethnography Museum, which has been split from the Russian Museum, is devoted to the cultures of the people of Russia, the former Soviet Union and Russian Empire.

Other notable museums include the Naval Museum hosted in the building of the former stock exchange and Zoological Museum, the Railway Museum, Museum of the Siege of Leningrad, Museum of the History of Saint Petersburg in the Peter and Paul Fortress and Artillery Museum, which in fact includes not only artillery items, but also a huge collection of other military equipment, uniform and decorations.